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Actin Polyclonal Antibody
 
Catalog No.
ST0096
Reactivity/
Applications
Human,Mouse,Rat/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
ACTG1/Actin cytoplasmic 2
Human_gene_id/
Human_swiss_prot_no
60/71/P60709/Q9BYX7/P63261
Mouse_gene_id/
Mouse_swiss_prot_no
11461/11465/
Rat_gene_id/
Rat_swiss_prot_no
81822/100361457/P60711/P63259
Immunogen
Synthesized peptide derived from Actin . at AA range: 300-380
Specificity
Actin Polyclonal Antibody detects endogenous levels of Actin protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin
Predicted_band(Da)/
Observed_band(KD)
41737/45
Content
Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000
Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000
Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
 
 
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