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Reactivity/ Applications |
Human,Mouse,Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
ACTG1/Actin cytoplasmic 2 |
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Human_gene_id/ Human_swiss_prot_no |
60/71/P60709/Q9BYX7/P63261 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
11461/11465/ |
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Rat_gene_id/ Rat_swiss_prot_no |
81822/100361457/P60711/P63259 |
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Immunogen |
Synthesized peptide derived from Actin . at AA range: 300-380 |
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Specificity |
Actin Polyclonal Antibody detects endogenous levels of Actin protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin |
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Predicted_band(Da)/ Observed_band(KD) |
41737/45 |
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Content |
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Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000 |
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Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000 |
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Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
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