|
Reactivity/
Applications |
Human,Mouse/WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
BRAF/Serine/threonine-protein kinase B-raf |
|
Human_gene_id/
Human_swiss_prot_no |
673/P15056 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
109880/P28028 |
|
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human Raf-B (phospho Ser446) |
|
Specificity |
Phospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 |
|
Predicted_band(Da)/
Observed_band(KD) |
84437/110 |
|
|
| Content |
|
Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1：1000 |
|
|
Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000 |
|
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