Caspase-9 (phospho Thr125) Polyclonal Antibody

Catalog No.

SP0598

Reactivity/
Applications

Human,Mouse,Rat/WB,IHC-p,ELISA

Size/Price

50ug/1000 100ug/1800

Gene_Name/
Protein_Name

CASP9/Caspase-9

Human_gene_id/
Human_swiss_prot_no

842/P55211

Rat_gene_id/
Rat_swiss_prot_no

58918/Q9JHK1

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human Caspase-9 (phospho Thr125)

Specificity

Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Other_name

CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Predicted_band(Da)/
Observed_band(KD)

46281/42

Content

Western Blot analysis of various cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1：500

Western Blot analysis of HeLa cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1：500