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Smad1 (phospho Ser465) Polyclonal Antibody
 
Catalog No.
SP0583
Reactivity/
Applications
Human,Mouse,Rat/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
SMAD1/Mothers against decapentaplegic homolog 1
Human_gene_id/
Human_swiss_prot_no
4086/Q15797
Mouse_gene_id/
Mouse_swiss_prot_no
17125/P70340
Rat_gene_id/
Rat_swiss_prot_no
/P97588
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Smad1 (phospho Ser465)
Specificity
Phospho-Smad1 (S465) Polyclonal Antibody detects endogenous levels of Smad1 protein only when phosphorylated at S465.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein
Predicted_band(Da)/
Observed_band(KD)
52260/60
Content
Western Blot analysis of various cells using Phospho-Smad1 (S465) Polyclonal Antibody
 
 
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