AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Data Sheet

Catalog No.

SP0575

Reactivity/
Applications

Human,Mouse,Rat,Monkey,Pig/IF WB,IHC-p,ELISA

Size/Price

50ug/1000 100ug/1800

Human_gene_id/
Human_swiss_prot_no

5562/5563/Q13131/P54646

Mouse_gene_id/
Mouse_swiss_prot_no

105787/108079/

Rat_gene_id/
Rat_swiss_prot_no

65248/78975/P54645/Q09137

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human AMPKα1/2 (phospho Thr183/172)

Specificity

Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Predicted_band(Da)/
Observed_band(KD)

62808/62320/63

Content

Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1：500

Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)