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Cyclin D1 (phospho Thr286) Polyclonal Antibody
 
Catalog No.
SP0297
Reactivity/
Applications
Human,Mouse,Rat/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
CCND1/G1/S-specific cyclin-D1
Human_gene_id/
Human_swiss_prot_no
595/P24385
Mouse_gene_id/
Mouse_swiss_prot_no
12443/P25322
Rat_gene_id/
Rat_swiss_prot_no
58919/P39948
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Cyclin D1 (phospho Thr286)
Specificity
Phospho-Cyclin D1 (T286) Polyclonal Antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at T286.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
Predicted_band(Da)/
Observed_band(KD)
33729/33
Content
Western Blot analysis of various cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500
Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500
 
 
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