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NFκB-p65 (phospho Ser529) Polyclonal Antibody
 
Catalog No.
SP0190
Reactivity/
Applications
Human,Mouse/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
RELA/Transcription factor p65
Human_gene_id/
Human_swiss_prot_no
5970/Q04206
Mouse_gene_id/
Mouse_swiss_prot_no
19697/Q04207
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human NFκB-p65 (phospho Ser529)
Specificity
Phospho-NFκB-p65 (S529) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S529.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Predicted_band(Da)/
Observed_band(KD)
60219/65
Content
Western Blot analysis of various cells using Phospho-NFκB-p65 (S529) Polyclonal Antibody diluted at 1:2000
 
 
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