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Reactivity/ Applications |
Human,Mouse,Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
HSPB1/Heat shock protein beta-1 |
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Human_gene_id/ Human_swiss_prot_no |
3315/P04792 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
15507/P14602 |
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Rat_gene_id/ Rat_swiss_prot_no |
24471/P42930 |
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Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human HSP27 (phospho Ser82) |
|
Specificity |
Phospho-HSP27 (S82) Polyclonal Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S82. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
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Predicted_band(Da)/ Observed_band(KD) |
22783/27 |
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Content |
|
Western Blot analysis of various cells using Phospho-HSP27 (S82) Polyclonal Antibody diluted at 1:1000 |
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