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GSK3α/β (phospho Tyr279/216) Polyclonal Antibody
 
Catalog No.
SP0123
Reactivity/
Applications
Human,Mouse,Rat/IF WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
GSK3A/GSK3B/Glycogen synthase kinase-3 alpha/beta
Human_gene_id/
Human_swiss_prot_no
2931/P49840/P49841
Mouse_gene_id/
Mouse_swiss_prot_no
606496/56637/
Rat_gene_id/
Rat_swiss_prot_no
50686/84027/P18265/P18266
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human GSK3α/β (phospho Tyr279/216)
Specificity
Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Predicted_band(Da)/
Observed_band(KD)
50981/51,46
Content
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
 
 
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