|
Reactivity/ Applications |
Human,Mouse,Rat/IF WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/ Protein_Name |
GSK3A/GSK3B/Glycogen synthase kinase-3 alpha/beta |
|
Human_gene_id/ Human_swiss_prot_no |
2931/P49840/P49841 |
|
Mouse_gene_id/ Mouse_swiss_prot_no |
606496/56637/ |
|
Rat_gene_id/ Rat_swiss_prot_no |
50686/84027/P18265/P18266 |
|
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human GSK3α/β (phospho Tyr279/216) |
|
Specificity |
Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B |
|
Predicted_band(Da)/ Observed_band(KD) |
50981/51,46 |
|
|
Content |
|
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) |
|
|