Connexin 43 (phospho Ser368) Polyclonal Antibody

Catalog No.

SP0071

Reactivity/
Applications

Human,Mouse,Rat/WB,IHC-p,ELISA

Size/Price

50ug/1000 100ug/1800

Gene_Name/
Protein_Name

GJA1/Gap junction alpha-1 protein

Human_gene_id/
Human_swiss_prot_no

2697/P17302

Mouse_gene_id/
Mouse_swiss_prot_no

14609/P23242

Rat_gene_id/
Rat_swiss_prot_no

24392/P08050

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human Connexin 43 (phospho Ser368)

Specificity

Phospho-Connexin 43 (S368) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S368.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Other_name

GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein

Predicted_band(Da)/
Observed_band(KD)

43008/43

Content

Western Blot analysis of various cells using Phospho-Connexin 43 (S368) Polyclonal Antibody diluted at 1：2000

Western Blot analysis of HuvEc cells using Phospho-Connexin 43 (S368) Polyclonal Antibody diluted at 1：2000