|
Reactivity/
Applications |
Human/WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
CDC25C/M-phase inducer phosphatase 3 |
|
Human_gene_id/
Human_swiss_prot_no |
995/P30307 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/P48967 |
|
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human Cdc25C (phospho Ser216) |
|
Specificity |
Phospho-Cdc25C (S216) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C |
|
Predicted_band(Da)/
Observed_band(KD) |
53365/53 |
|
|
| Content |
|
Western Blot analysis of various cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1：1000 |
|
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Western Blot analysis of HuvEc cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1：1000 |
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