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Akt2 (phospho Ser474) Polyclonal Antibody
 
Catalog No.
SP0009
Reactivity/
Applications
Human,Mouse,Rat/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
AKT2/RAC-beta serine/threonine-protein kinase
Human_gene_id/
Human_swiss_prot_no
208/P31751
Mouse_gene_id/
Mouse_swiss_prot_no
11652/Q60823
Rat_gene_id/
Rat_swiss_prot_no
25233/P47197
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Akt2 (phospho Ser474)
Specificity
Phospho-Akt2 (S474) Polyclonal Antibody detects endogenous levels of Akt2 protein only when phosphorylated at S474.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
Predicted_band(Da)/
Observed_band(KD)
55769/60
Content
Western Blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500
Western Blot analysis of 293 cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500
 
 
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