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Reactivity/
Applications |
Human,Mouse,Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
AKT1/RAC-alpha serine/threonine-protein kinase |
|
Human_gene_id/
Human_swiss_prot_no |
207/P31749 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
11651/P31750 |
|
Rat_gene_id/
Rat_swiss_prot_no |
24185/P47196 |
|
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human Akt1 (phospho Thr450) |
|
Specificity |
Phospho-Akt1 (T450) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha |
|
Predicted_band(Da)/
Observed_band(KD) |
55686/55 |
|
|
| Content |
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Western Blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000 |
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Western Blot analysis of 453 cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000 |
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Western Blot analysis of HEPG2 KB A549 using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000 |
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