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Reactivity/
Applications |
H, M, R/IF WB IHC-p ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
E2F1 RBBP3/E2F transcription factor 1 |
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Human_gene_id/
Human_swiss_prot_no |
1869/Q01094 |
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Mouse_gene_id/
Mouse_swiss_prot_no |
13555/Q61501 |
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Rat_gene_id/
Rat_swiss_prot_no |
/O09139 |
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Immunogen |
Synthetic peptide from human protein at AA range: 100-170 |
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Specificity |
The antibody detects endogenous E2F-1 protein |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
IF: 1:50-200 WB 1:500-2000, ELISA 1：10000-20000 IHC 1:50-300 |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Predicted_band(Da)/
Observed_band(KD) |
46920/47 |
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| Content |
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Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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