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Reactivity/
Applications |
Human, Mouse, Rat/IF WB IHC-p ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
EP300/Histone acetyltransferase p300 |
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Human_gene_id/
Human_swiss_prot_no |
2033/Q09472 |
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Mouse_gene_id/
Mouse_swiss_prot_no |
/B2RWS6 |
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Immunogen |
Synthesized peptide derived from the Internal region of human p300. |
|
Specificity |
p300 Polyclonal Antibody detects endogenous levels of p300 protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300 |
|
Predicted_band(Da)/
Observed_band(KD) |
264161/300 |
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| Content |
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Immunofluorescence analysis of rat-kidney tissue. 1,p300 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-kidney tissue. 1,p300 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,p300 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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