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Reactivity/ Applications |
H, R, M/IF IHC-p |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
MAPK3/MAPK3 |
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Human_gene_id/ Human_swiss_prot_no |
5594/P27361 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
/Q63844 |
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Rat_gene_id/ Rat_swiss_prot_no |
/P21708 |
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Immunogen |
Recombinant Protein of ERK1 of MAPK3 |
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Specificity |
ERK1 protein detects endogenous levels of MAPK3 |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
IF: 1:50-200 IHC 1:100-200 |
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Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Predicted_band(Da)/ Observed_band(KD) |
/44 |
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Content |
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Immunofluorescence analysis of rat-spleen tissue. 1,ERK1 Mouse Monoclonal Antibody(5E9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,ERK1 Mouse Monoclonal Antibody(5E9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using ERK1 Mouse mAb diluted at 1:200. |
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