|
Reactivity/
Applications |
H, R, M/WB, IF,IHC-p |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
/Ubiquitin |
|
Human_gene_id/
Human_swiss_prot_no |
7314/PAN |
|
Immunogen |
Synthetic Peptide of Ubiquitin |
|
Specificity |
Ubiquitin protein detects endogenous levels of Ubiquitin |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
WB 1:1000-2000, IHC 1:100-200 IF 1:200 |
|
Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Predicted_band(Da)/
Observed_band(KD) |
/N/A |
|
|
| Content |
|
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
|
|
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
|
|
Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
|
| |
