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Reactivity/ Applications |
H, R, M/WB IHC-p |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
AKT1/RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha) |
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Human_gene_id/ Human_swiss_prot_no |
207/P31749 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
/P31750 |
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Rat_gene_id/ Rat_swiss_prot_no |
/P47196 |
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Immunogen |
Recombinant Protein of Akt1 |
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Specificity |
Antibody can detects endogenous Akt1 protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha) |
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Predicted_band(Da)/ Observed_band(KD) |
/60 |
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Content |
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Western blot analysis of 1) Hela, 2)3T3, 3) PC12 with Akt1 Rabbit pAb diluted at 1:2,000.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Akt1 Rabbit pAb diluted at 1:200. |
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