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Akt1 Polyclonal Antibody
 
Catalog No.
SM3461
Reactivity/
Applications
H, R, M/WB IHC-p
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
AKT1/RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha)
Human_gene_id/
Human_swiss_prot_no
207/P31749
Mouse_gene_id/
Mouse_swiss_prot_no
/P31750
Rat_gene_id/
Rat_swiss_prot_no
/P47196
Immunogen
Recombinant Protein of Akt1
Specificity
Antibody can detects endogenous Akt1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha)
Predicted_band(Da)/
Observed_band(KD)
/60
Content
Western blot analysis of 1) Hela, 2)3T3, 3) PC12 with Akt1 Rabbit pAb diluted at 1:2,000.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Akt1 Rabbit pAb diluted at 1:200.
 
 
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