|
Reactivity/
Applications |
Human, Mouse, Rat/WB IHC-p |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
SMAD3/Mothers against decapentaplegic homolog 3 |
|
Human_gene_id/
Human_swiss_prot_no |
4088/P84022 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/Q8BUN5 |
|
Rat_gene_id/
Rat_swiss_prot_no |
/P84025 |
|
Immunogen |
Synthesized peptide derived from human Smad3 around the non-phosphorylation site of S213. |
|
Specificity |
The antibody detects endogenous Smad3 protein. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
Storage_stability |
-20°C/1 year |
|
Other_name |
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
|
Predicted_band(Da)/
Observed_band(KD) |
/50 |
|
|
| Content |
|
Western blot analysis of 1) A431, 2) Hela, 3) C2C12, 4) 3T3, 5) PC12, 6) Rat Brain Tissue using Smad3 Polyclonal Antibody.. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
|
|
Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using Smad3 Polyclonal Antibody. |
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