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Reactivity/ Applications |
Human, Mouse, Rat/WB IHC-p |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
HSPB1/Heat shock protein beta-1 |
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Human_gene_id/ Human_swiss_prot_no |
3315/P04792 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
/P14602 |
|
Rat_gene_id/ Rat_swiss_prot_no |
/P42930 |
|
Immunogen |
Synthesized peptide derived from human HSP27 around the non-phosphorylation site of S15. |
|
Specificity |
The antibody detects endogenous HSP27 protein. |
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Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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|
Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
|
Predicted_band(Da)/ Observed_band(KD) |
/27 |
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| Content |
|
Western blot analysis of 1) Hela, 2) C2C12, 3) Mouse Brain, 4) Rat Brain using HSP27 Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSP27 Polyclonal Antibody. |
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