Histone H4 (Acetyl Lys16) Polyclonal Antibody

Data Sheet

Catalog No.

SM3317

Reactivity/
Applications

H, M, R/IF WB IHC-p

Size/Price

50ug/1000 100ug/1800

Gene_Name/
Protein_Name

HIST1H4A/Histone H4

Human_gene_id/
Human_swiss_prot_no

121504/P62805

Mouse_gene_id/
Mouse_swiss_prot_no

100041230/P62806

Rat_gene_id/
Rat_swiss_prot_no

100360950/P62804

Immunogen

Synthetic Peptide of Histone H4 (Acetyl Lys16)

Specificity

The antibody detects endogenous Histone H3 (Acetyl Lys16) protein.

Formulation

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Source

Rabbit

Dilution

IF: 1:50-200 WB: 1:1000-2000 IHC 1:50-300

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage_stability

-20°C/1 year

Other_name

HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK; HIST2H4A; H4/

Predicted_band(Da)/
Observed_band(KD)

11367/14

Content

Immunofluorescence analysis of human-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.