|
Reactivity/
Applications |
H/WB IHC-p , IF, |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
IDE/Insulin-degrading enzyme |
|
Human_gene_id/
Human_swiss_prot_no |
3416/P14735 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/Q9JHR7 |
|
Rat_gene_id/
Rat_swiss_prot_no |
25700/P35559 |
|
Immunogen |
Synthetic Peptide of IDE |
|
Specificity |
The antibody detects endogenous IDE proteins. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
|
|
Dilution |
WB: 1:1000 IF 1:200 IHC 1:50-300 |
|
Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
|
Storage_stability |
-20°C/1 year |
|
Other_name |
IDE; Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin |
|
Predicted_band(Da)/
Observed_band(KD) |
/118 |
|
|
| Content |
|
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,IDE Monoclonal Antibody(3H4) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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|
Immunofluorescence analysis of Human-breast tissue. 1,IDE Monoclonal Antibody(3H4)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) HepG2, diluted at 1:2000 |
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