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Reactivity/ Applications |
H/WB IHC-p , IF, IP |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
KRT17/Keratin type I cytoskeletal 17 |
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Human_gene_id/ Human_swiss_prot_no |
3872/Q04695 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
16667/Q9QWL7 |
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Rat_gene_id/ Rat_swiss_prot_no |
287702/Q6IFU8 |
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Immunogen |
Synthetic Peptide of CK17 |
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Specificity |
The antibody detects CK17 endogenous proteins. |
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Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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Dilution |
WB: 1:1000 IP:1:200 IF 1:200 IHC 1:50-300 |
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Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
KRT17; Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17 |
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Predicted_band(Da)/ Observed_band(KD) |
48106/48 |
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Content |
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000. |
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