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CK17 Monoclonal Antibody(10A1)
 
Catalog No.
SM3081
Reactivity/
Applications
H/WB IHC-p , IF, IP
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
KRT17/Keratin type I cytoskeletal 17
Human_gene_id/
Human_swiss_prot_no
3872/Q04695
Mouse_gene_id/
Mouse_swiss_prot_no
16667/Q9QWL7
Rat_gene_id/
Rat_swiss_prot_no
287702/Q6IFU8
Immunogen
Synthetic Peptide of CK17
Specificity
The antibody detects CK17 endogenous proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Mouse
Dilution
WB: 1:1000 IP:1:200 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability
-20°C/1 year
Other_name
KRT17; Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17
Predicted_band(Da)/
Observed_band(KD)
48106/48
Content
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000.
 
 
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