|
Reactivity/
Applications |
H/IF WB IHC-p , IP |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
CA9/Carbonic anhydrase 9 |
|
Human_gene_id/
Human_swiss_prot_no |
768/Q16790 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
230099/Q8VHB5 |
|
Immunogen |
Synthetic Peptide of CA IX |
|
Specificity |
The antibody detects endogenous CA IX proteins. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
|
|
Dilution |
IF: 1:50-200 WB: 1:3000 IP:1:200 IHC 1:50-300 |
|
Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
|
Storage_stability |
-20°C/1 year |
|
Other_name |
CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 |
|
Predicted_band(Da)/
Observed_band(KD) |
49698/35-38 |
|
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| Content |
|
Immunofluorescence analysis of human-liver-cancer tissue. 1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western blot analysis of 1) Hela, 2) 293T, diluted at 1:5000. |
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