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Reactivity/
Applications |
N/A/WB, IP, IF |
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Size/Price |
50ug/1000 100ug/1800 |
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Immunogen |
Synthetic Peptide of VSV-G-Tag |
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Specificity |
The antibody detects C-terminal, internal, and N-terminal VSV-G fusion proteins. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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Dilution |
WB: 1:5000 IP: 1:200 IF: 1:1000 |
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Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
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Storage_stability |
-20°C/1 year |
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| Content |
|
1ug VSV-G fusion protein+ Primary antibody dilution at
1) 1:5000
2) 1:10000 |
|
|
IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G) |
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IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1: 5000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with VSV-G-tag fusion protein
3) IP (untransfted 293+anti-VSV-G mAb+Protein G agarose)
4) IP (transfected 293+ normal Mouse |
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