|
Reactivity/
Applications |
N/A/WB, IP, IF |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Immunogen |
Synthetic peptide:HHHHHH conjugated to KLH. |
|
Specificity |
The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
|
|
Dilution |
WB: 1:3000 IP: 1:200 IF: 1:1000 |
|
Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
|
Storage_stability |
-20°C/1 year |
|
|
| Content |
|
2ug His fusion protein+ Primary antibody dilution at
1) 1:5000
2) 1:10000 |
|
|
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His) |
|
|
IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with His-tag fusion protein
3) IP (untransfected 293+anti-His mAb+ Protein G agarose)
4) IP (transfected 293+ normal Mouse IgG |
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| |
