|
Reactivity/
Applications |
N/A/WB, IP, IF |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Immunogen |
Synthetic Peptide of Flag-Tag |
|
Specificity |
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
|
Formulation |
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
|
|
Dilution |
WB: 1:5000 IP: 1:200 IF: 1:2000 |
|
Purification |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
|
Storage_stability |
-20°C/1 year |
|
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| Content |
|
1ug Flag fusion protein+ Primary antibody dilution at 1:10000 |
|
|
IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag) |
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|
IP antibody use: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with Flag-tag fusion protein
3) IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4) IP (transfected 293+anti- Flag mA |
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