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NFκB-p65 (Acetyl Lys221) Polyclonal Antibody
 
Catalog No.
SK0065
Reactivity/
Applications
Human, Mouse, Rat/WB ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
RELA/Transcription factor p65
Human_gene_id/
Human_swiss_prot_no
5970/Q04206
Mouse_gene_id/
Mouse_swiss_prot_no
19697/Q04207
Immunogen
Synthesized acetyl-peptide derived from the Internal region of human NFκB-p65 around the acetylation site of K221.
Specificity
Acetyl-NFκB-p65 (K221) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylation at K221.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Predicted_band(Da)/
Observed_band(KD)
60219/65
Content
Western Blot analysis of HepG2-UV, mouse brain cells using Acetyl-NFκB-p65 (K221) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of HEPG2-UV cells using Acetyl-NFκB-p65 (K221) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
 
 
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