Nrf2 (Acetyl Lys599) Polyclonal Antibody

Data Sheet

Catalog No.

SK0063

Reactivity/
Applications

Human, Mouse, Rat/WB,IHC-p,ELISA

Size/Price

50ug/1000 100ug/1800

Gene_Name/
Protein_Name

NFE2L2/Nuclear factor erythroid 2-related factor 2

Human_gene_id/
Human_swiss_prot_no

4780/Q16236

Mouse_gene_id/
Mouse_swiss_prot_no

18024/Q60795

Rat_gene_id/
Rat_swiss_prot_no

83619/O54968

Immunogen

Synthesized acetyl-peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599.

Specificity

Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when acetylation at K599.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Other_name

NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

Predicted_band(Da)/
Observed_band(KD)

75-100/75-100

Content

Western Blot analysis of A549 cells using Acetyl-Nrf2 (K599) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100