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Reactivity/
Applications |
Human, Mouse, Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
HMGB1/High mobility group protein B1 |
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Human_gene_id/
Human_swiss_prot_no |
3146/P09429 |
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Mouse_gene_id/
Mouse_swiss_prot_no |
100862258/P63158 |
|
Rat_gene_id/
Rat_swiss_prot_no |
25459/P63159 |
|
Immunogen |
Synthesized acetyl-peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12. |
|
Specificity |
Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when acetylation at K12. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
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Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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|
Storage_stability |
-20°C/1 year |
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Other_name |
HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 |
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Predicted_band(Da)/
Observed_band(KD) |
24894/25 |
|
|
| Content |
|
Western Blot analysis of NIH-3T3, HepG2-UV cells using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
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