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p53 (Acetyl Lys372) Polyclonal Antibody
 
Catalog No.
SK0036
Reactivity/
Applications
Human/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
TP53/Cellular tumor antigen p53
Human_gene_id/
Human_swiss_prot_no
7157/P04637
Mouse_gene_id/
Mouse_swiss_prot_no
/P02340
Immunogen
Synthesized acetyl-peptide derived from the human p53 around the acetylation site of K372.
Specificity
Acetyl-p53 (K372) Polyclonal Antibody detects endogenous levels of p53 protein only when acetylated at K372.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Predicted_band(Da)/
Observed_band(KD)
43653/53
Content
Western Blot analysis of Jurkat cells using Acetyl-p53 (K372) Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of 293T cells using Acetyl-p53 (K372) Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
 
 
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