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Reactivity/
Applications |
Human/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
TP53/Cellular tumor antigen p53 |
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Human_gene_id/
Human_swiss_prot_no |
7157/P04637 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/P02340 |
|
Immunogen |
Synthesized acetyl-peptide derived from the human p53 around the acetylation site of K370. |
|
Specificity |
Acetyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when acetylated at K370. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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|
Storage_stability |
-20°C/1 year |
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Other_name |
TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 |
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Predicted_band(Da)/
Observed_band(KD) |
43653/53 |
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| Content |
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Western Blot analysis of Jurkat cells using Acetyl-p53 (K370) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Western Blot analysis of AD293 cells using Acetyl-p53 (K370) Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100 |
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