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Reactivity/
Applications |
Human, Mouse, Rat/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
RELA/Transcription factor p65 |
|
Human_gene_id/
Human_swiss_prot_no |
5970/Q04206 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
19697/Q04207 |
|
Immunogen |
Synthesized acetyl-peptide derived from the human NFκB-p65 around the acetylation site of K218. |
|
Specificity |
Acetyl-NFκB-p65 (K218) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K218. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
|
Predicted_band(Da)/
Observed_band(KD) |
60219/60 |
|
|
| Content |
|
Western Blot analysis of Jurkat, K562 cells using Acetyl-NFκB-p65 (K218) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Western Blot analysis of MOUSE-BRAIN cells using Acetyl-NFκB-p65 (K218) Polyclonal Antibody diluted at 1：1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100 |
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