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Cytokeratin 8 (Acetyl Lys483) Polyclonal Antibody
 
Catalog No.
SK0031
Reactivity/
Applications
Human/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
KRT8/Keratin, type II cytoskeletal 8
Human_gene_id/
Human_swiss_prot_no
3856/P05787
Mouse_gene_id/
Mouse_swiss_prot_no
/P11679
Immunogen
Synthesized acetyl-peptide derived from the human Cytokeratin 8 around the acetylation site of K483.
Specificity
Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein only when acetylated at K483.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Predicted_band(Da)/
Observed_band(KD)
53573/53
Content
Western Blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
 
 
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