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Reactivity/ Applications |
Human/WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
APEX1/DNA-(apurinic or apyrimidinic site) lyase |
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Human_gene_id/ Human_swiss_prot_no |
328/P27695 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
/P28352 |
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Immunogen |
Synthesized acetyl-peptide derived from the human Ref-1 around the acetylation site of K6. |
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Specificity |
Acetyl-Ref-1 (K6) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K6. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1 |
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Predicted_band(Da)/ Observed_band(KD) |
35555/35 |
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Content |
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Western Blot analysis of A549 cells using Acetyl-Ref-1 (K6) Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
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Western Blot analysis of A549 cells using Acetyl-Ref-1 (K6) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100 |
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