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Ref-1 (Acetyl Lys6) Polyclonal Antibody
 
Catalog No.
SK0022
Reactivity/
Applications
Human/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
APEX1/DNA-(apurinic or apyrimidinic site) lyase
Human_gene_id/
Human_swiss_prot_no
328/P27695
Mouse_gene_id/
Mouse_swiss_prot_no
/P28352
Immunogen
Synthesized acetyl-peptide derived from the human Ref-1 around the acetylation site of K6.
Specificity
Acetyl-Ref-1 (K6) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K6.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1
Predicted_band(Da)/
Observed_band(KD)
35555/35
Content
Western Blot analysis of A549 cells using Acetyl-Ref-1 (K6) Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of A549 cells using Acetyl-Ref-1 (K6) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
 
 
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