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Histone H2B (Acetyl Lys5) Polyclonal Antibody
 
Catalog No.
SK0003
Reactivity/
Applications
Human,Mouse/WB,IHC-p,IF,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
H2BFS/Histone H2B type F-S
Human_gene_id/
Human_swiss_prot_no
54145/P57053
Immunogen
Synthesized acetyl-peptide derived from Histone H2B (Acetyl Lys5) . at AA range: 1-80
Specificity
Acetyl-Histone H2B (K5) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K5.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s
Predicted_band(Da)/
Observed_band(KD)
13944/18
Content
Western Blot analysis of various cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
 
 
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