p53 (Di Methyl Lys370) Polyclonal Antibody

Data Sheet

Catalog No.

SH0007

Reactivity/
Applications

Human/IF WB,IHC-p,ELISA

Size/Price

50ug/1000 100ug/1800

Gene_Name/
Protein_Name

TP53/Cellular tumor antigen p53

Human_gene_id/
Human_swiss_prot_no

7157/P04637

Mouse_gene_id/
Mouse_swiss_prot_no

/P02340

Immunogen

Synthesized peptide derived from human p53 around the di-methylation site of K370.

Specificity

Di-Methyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when di-methylated at K370.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Rabbit

Dilution

IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Other_name

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Predicted_band(Da)/
Observed_band(KD)

43653/53

Content

Immunofluorescence analysis of human-breast-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B