|
Reactivity/
Applications |
N/A/WB,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Immunogen |
6X His synthetic peptide conjugated to KLH. |
|
Specificity |
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide. |
|
|
Dilution |
Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag |
|
Predicted_band(Da)/
Observed_band(KD) |
/15 |
|
|
| Content |
|
Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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