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Caspase-7 Polyclonal Antibody
 
Catalog No.
SC0032
Reactivity/
Applications
Human/WB IHC-p , IF,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
CASP7/Caspase-7
Human_gene_id/
Human_swiss_prot_no
840/P55210
Mouse_gene_id/
Mouse_swiss_prot_no
/P97864
Immunogen
Synthesized peptide derived from Caspase-7 . at AA range: 160-240
Specificity
Caspase-7 Polyclonal Antibody detects endogenous levels of Caspase-7 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
WB 1:500-2000, IF 1:50-300, IHC 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3
Predicted_band(Da)/
Observed_band(KD)
34277/34
Content
Western Blot analysis of various cells using Caspase-7 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1,Caspase-7 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
 
 
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