Topoisomerase IIα (ABT-TOPO) mouse mAb

Data Sheet

Catalog No.

SM6594

Reactivity/
Applications

Human/IHC-p,WB,IF

Size/Price

50ul/1400 100ul/2600

Gene_Name/
Protein_Name

TOP2A TOP2/DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme)

Human_gene_id/
Human_swiss_prot_no

7153/P11388

Immunogen

Synthesized peptide derived from human Topoisomerase IIα

Specificity

This antibody detects endogenous levels of human Topoisomerase IIα

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Mouse/IgG1, Kappa

Dilution

IHC-p 1:100-500，WB 1:500-2000，IF 1:500-200

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Content

Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Heat-induced epitope retrieval (HIER) was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).