PIK3CA H1047R (ABT-PI3.H1047R) mouse mAb

Data Sheet

Catalog No.

SM6560

Reactivity/
Applications

Human/IHC-p,WB,IF

Size/Price

50ul/1400 100ul/2600

Gene_Name/
Protein_Name

PIK3CA/Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase subunit alpha) (PI3K-alpha) (PI3Kalpha) (PtdIns-3-kinase subunit alpha) (EC 2.7.1.153) (Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subu

Human_gene_id/
Human_swiss_prot_no

5290/P42336

Immunogen

Synthesized peptide derived from human PIK3CA H1047R

Specificity

This antibody detects endogenous levels of human PIK3CA H1047R

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Mouse/IgG3, Kappa

Dilution

IHC-p 1:100-500，WB 1:500-2000，IF 1:500-200

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Storage_stability

-20°C/1 year

Content

Immunohistochemical analysis of paraffin-embedded Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Heat-induced epitope retrieval (HIER) was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of PIK3CAAntibody at 1:1000 dilution.