|
Reactivity/
Applications |
Human, Mouse, Rat/WB IHC-p ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
SPARC/SPARC |
|
Human_gene_id/
Human_swiss_prot_no |
6678/P09486 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
20692/P07214 |
|
Rat_gene_id/
Rat_swiss_prot_no |
24791/P16975 |
|
Immunogen |
Synthesized peptide derived from SPARC . at AA range: 141-190 |
|
Specificity |
SPARC Polyclonal Antibody detects endogenous levels of SPARC protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine |
|
Predicted_band(Da)/
Observed_band(KD) |
33330/34 |
|
|
| Content |
|
Western Blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody.. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
|
|
Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100 |
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| |
