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Histone H2A Polyclonal Antibody
 
Catalog No.
ST5500
Reactivity/
Applications
Human, Mouse, Rat/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
HIST1H2AG/HIST1H2AI/HIST1H2AK/HIST1H2AL/HIST1H2AM/Histone H2A type 1
Human_gene_id/
Human_swiss_prot_no
8329/8330/8332/8336/8969/P0C0S8
Mouse_gene_id/
Mouse_swiss_prot_no
319164/P22752
Rat_gene_id/
Rat_swiss_prot_no
/P02262
Immunogen
Synthesized peptide derived from the Internal region of human Histone H2A.
Specificity
Histone H2A Polyclonal Antibody detects endogenous levels of Histone H2A protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p
Predicted_band(Da)/
Observed_band(KD)
14091/15
Content
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Histone H2A Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of HeLa cells using Histone H2A Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
 
 
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