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gp91-phox Polyclonal Antibody
 
Catalog No.
ST5383
Reactivity/
Applications
Human/WB,IHC-p,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
CYBB/Cytochrome b-245 heavy chain
Human_gene_id/
Human_swiss_prot_no
1536/P04839
Mouse_gene_id/
Mouse_swiss_prot_no
/Q61093
Immunogen
Synthesized peptide derived from gp91-phox . at AA range: internal
Specificity
gp91-phox Polyclonal Antibody detects endogenous levels of gp91-phox protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase
Predicted_band(Da)/
Observed_band(KD)
65336/70
Content
Western Blot analysis of K562 cells using gp91-phox Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of HEPG2 using gp91-phox Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100
 
 
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