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Reactivity/ Applications |
Human/IF WB,IHC-p,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/ Protein_Name |
MMP9/Matrix metalloproteinase-9 |
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Human_gene_id/ Human_swiss_prot_no |
4318/P14780 |
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Mouse_gene_id/ Mouse_swiss_prot_no |
/P41245 |
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Immunogen |
Synthesized peptide derived from MMP-9 . at AA range: INTERNAL |
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Specificity |
MMP-9 Polyclonal Antibody detects endogenous levels of MMP-9 protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Dilution |
IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Storage_stability |
-20°C/1 year |
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Other_name |
MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB |
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Predicted_band(Da)/ Observed_band(KD) |
78427/80 |
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Content |
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Immunofluorescence analysis of human-liver tissue. 1,MMP-9 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of human-kidney tissue. 1,MMP-9 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,MMP-9 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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