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RUNX2 Polyclonal Antibody
 
Catalog No.
ST5356
Reactivity/
Applications
Human,Mouse,Rat/IF WB,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
RUNX2/Runt-related transcription factor 2
Human_gene_id/
Human_swiss_prot_no
860/Q13950
Mouse_gene_id/
Mouse_swiss_prot_no
12393/Q08775
Rat_gene_id/
Rat_swiss_prot_no
/Q9Z2J9
Immunogen
Synthesized peptide derived from RUNX2 . at AA range: 191-240
Specificity
RUNX2 Polyclonal Antibody detects endogenous levels of RUNX2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
RUNX2; AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding prote
Predicted_band(Da)/
Observed_band(KD)
56648/56
Content
Immunofluorescence analysis of human-stomach tissue. 1,RUNX2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western Blot analysis of K562 cells using RUNX2 Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
 
 
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