|
Reactivity/
Applications |
Human/WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
IL6/Interleukin-6 |
|
Human_gene_id/
Human_swiss_prot_no |
3569/P05231 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/P08505 |
|
Immunogen |
Synthesized peptide derived from IL-6 . at AA range: INTERNAL |
|
Specificity |
IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
|
Predicted_band(Da)/
Observed_band(KD) |
21~30/23 |
|
|
| Content |
|
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,IL-6 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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|
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,IL-6 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of K562 cells using IL-6 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
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