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Nrf2 Polyclonal Antibody
 
Catalog No.
ST5337
Reactivity/
Applications
Human, Mouse, Rat/IF WB IHC-p ,ELISA
Size/Price
50ug/1000 100ug/1800
Gene_Name/
Protein_Name
NFE2L2/Nuclear factor erythroid 2-related factor 2
Human_gene_id/
Human_swiss_prot_no
4780/Q16236
Mouse_gene_id/
Mouse_swiss_prot_no
18024/Q60795
Rat_gene_id/
Rat_swiss_prot_no
83619/O54968
Immunogen
Synthesized peptide derived from human Nrf2 around the non-acetylation site of K599.
Specificity
Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Rabbit
Dilution
IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage_stability
-20°C/1 year
Other_name
NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2
Predicted_band(Da)/
Observed_band(KD)
75-100/75-100
Content
Immunofluorescence analysis of human-stomach tissue. 1,Nrf2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,Nrf2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Nrf2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
 
 
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