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Reactivity/
Applications |
Human, Mouse, Rat/IF WB IHC-p ,ELISA |
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Size/Price |
50ug/1000 100ug/1800 |
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Gene_Name/
Protein_Name |
CASP3/Caspase-3 |
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Human_gene_id/
Human_swiss_prot_no |
836/P42574 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
12367/P70677 |
|
Rat_gene_id/
Rat_swiss_prot_no |
25402/P55213 |
|
Immunogen |
Synthesized peptide derived from Caspase-3 at AA range: 171-220 |
|
Specificity |
Caspase-3 Polyclonal Antibody detects endogenous levels of Caspase-3 protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
IF: 1:50-200 WB 1:500-2000, ELISA 1：10000-20000 IHC 1:50-300 |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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|
Storage_stability |
-20°C/1 year |
|
Other_name |
CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1 |
|
Predicted_band(Da)/
Observed_band(KD) |
31608/35 |
|
|
| Content |
|
Immunofluorescence analysis of human-lung tissue. 1,Caspase-3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-lung tissue. 1,Caspase-3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) |
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