|
Reactivity/
Applications |
Human/IF WB,IHC-p,ELISA |
|
Size/Price |
50ug/1000 100ug/1800 |
|
Gene_Name/
Protein_Name |
MDM2/E3 ubiquitin-protein ligase Mdm2 |
|
Human_gene_id/
Human_swiss_prot_no |
4193/Q00987 |
|
Mouse_gene_id/
Mouse_swiss_prot_no |
/P23804 |
|
Immunogen |
Synthesized peptide derived from E3 ubiquitin-protein ligase Mdm2 at AA range: 381-430 |
|
Specificity |
MDM2 Polyclonal Antibody detects endogenous levels of MDM2 protein. |
|
Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
|
|
Dilution |
IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
|
Storage_stability |
-20°C/1 year |
|
Other_name |
MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2 |
|
Predicted_band(Da)/
Observed_band(KD) |
55233/55 |
|
|
| Content |
|
Western Blot analysis of mouse liver cells using MDM2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody（catalog#：RS0002) was diluted at 1:20000 |
|
|
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100 |
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